| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein O3;AF6q21 protein;Forkhead in rhabdomyosarcoma-like 1;FOXO3;FKHRL1, FOXO3A; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FOXO3A recombinant protein (Position: Q471-G673). Human FOXO3A shares 97% amino acid (aa) sequence identity with mouse FOXO3A. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets forkhead box protein O3 (gene FOXO3) in human and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 71.3 kDa, and the supplier reports an observed band at about 80–90 kDa.
FOXO3 is a forkhead transcription factor inhibited by AKT phosphorylation downstream of insulin and growth factor signaling. When active in the nucleus, it drives genes for autophagy, oxidative stress resistance, cell cycle arrest and apoptosis. FOXO3 is studied in stem cell maintenance, muscle atrophy, cancer and aging.
| Target | Forkhead box protein O3 (gene FOXO3; UniProt O43524, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 71.3 kDa |
| Observed MW | 80–90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human 293T cells, human MCF-7 cells, human Jurkat cells, human HeLa cells, rat lung tissue, rat ovary tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human pancreatic ductal adenocarcinoma tissue, human colorectal adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human FOXO3A fragment (Gln471–Gly673), expressed in E. coli. Sequence identity with the mouse ortholog: 97%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places forkhead box protein O3 in the cytoplasm, nucleus, mitochondrion matrix and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huan Deng et al. (2025) Chondroitin sulfate A-selenium nanoparticles protect chondrocytes from T-2 toxin-induced oxidative stress and mitochondrial dysfunction through activating autophagy by the SIRT1-AMPK-FOXO3 pathway. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2025.118797
Siegfried Labeit et al. (2021) Regulation of Glucose Metabolism by MuRF1 and Treatment of Myopathy in Diabetic Mice with Small Molecules Targeting MuRF1. International Journal of Molecular Sciences. 10.3390/ijms22042225
Tang Han et al. (2022) Arbutin Protects Retinal Pigment Epithelium Against Oxidative Stress by Modulating SIRT1/FOXO3a/PGC-1α/β Pathway. Frontiers in Genetics. 10.3389/fgene.2022.922807
Liu Longfei et al. (2024) Genistein Induces Ferroptosis in Colorectal Cancer Cells via FoxO3/SLC7A11/GPX4 Signaling Pathway. Journal of Cancer. 10.7150/jca.95775
