| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ornithine carbamoyltransferase, mitochondrial;2.1.3.3;Ornithine transcarbamylase;OTCase;OTC; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FOXP1, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FOXP1 Antibody Picoband® is an antibody for FOXP1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: FOXP1 (Ornithine carbamoyltransferase, mitochondrial); UniProt: Q9H334
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 82 kDa, calculated 39935 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-FOXP1 Antibody Picoband® catalog # A00723.
Biological background
Biological context: May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
Expression and localization notes: cellular localization: Mitochondrion matrix., tissue context: Mainly expressed in liver and intestinal mucosa..
Common research applications
- Western blotting (WB): Compare FOXP1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of FOXP1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify FOXP1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: FOXP1 (forkhead box P1) is a gene that is necessary for the proper development of the brain and lung in mammals. It is a member of the large FOX family of transcription factors. This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene (s). Alternative splicing results in multiple transcript variants encoding different isoforms. It was shown that the embryonic stem cell (ESC)-specific isoform of FOXP1 stimulates the expression of transcription factor genes required for pluripotency, including OCT4, NANOG, NR5A2, and GDF3, while concomitantly repressing genes required for ESC differentiation. This isoform also promotes the maintenance of ESC pluripotency and contributes to efficient reprogramming of somatic cells into induced pluripotent stem cells. These results reveal a pivotal role for an Alternative splicing event in the regulation of pluripotency through the control of critical ESC-specific transcriptional programs (2).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Mitochondrion matrix.
- Tissue details: Mainly expressed in liver and intestinal mucosa.
- Research category: Biochemicals,Chemical Type,Neuroscience,Neurotransmission,Obesity,Receptors / Channels
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
