Anti-FRA2 Rabbit Monoclonal Antibody

Overview

This product is an anti-FOSL2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24F44; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP, Flow (as provided in the source record). Boster Bio Anti-FRA2 Rabbit Monoclonal Antibody catalog # M02615-2. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: FOSL2 (Calcium/calmodulin-dependent protein kinase type II subunit delta).
• Antibody format: Monoclonal; clone 24F44; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

FOSL2 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca (2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca (2+) influx into the myocyte, Ca (2+) release from the sarcoplasmic reticulum (SR), SR Ca (2+) uptake and Na (+) and K (+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca (2+) release via phosphorylation of RYR2 Ca (2+) channel on 'Ser- 2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca (2+) influx to myocytes by binding and phosphorylating the L-type Ca (2+) channel subunit beta-2 CACNB2. In addition to Ca (2+) channels, can target and regulate the cardiac sarcolemmal Na (+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca (2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca (2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor- coupling factor. . Reported cellular localization context: Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Sarcoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Tissue expression notes (as provided): Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle. .

Research relevance and current trends

• Research context keywords from the source record include: Calcium Signaling,Calmodulin Pathway,Cardiovascular,Hypertrophy,Neuroscience,Neurotransmission,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathway.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate FOSL2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect FOSL2 expression by Western blot in cell or tissue lysates, Quantify FOSL2-positive cells by flow cytometry in single-cell suspensions, Enrich FOSL2 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 45 kDa; calculated MW: 56369 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 45 kDa
• Cellular localization (provided): Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Sarcoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side .
• Tissue details (provided): Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.