| Field | Specification |
|---|---|
| Target | |
| Alternative names | Follitropin subunit beta;Follicle-stimulating hormone beta subunit;FSH-B;FSH-beta;Follitropin beta chain;FSHB; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FSH beta recombinant protein (Position: N19-E129). Human FSH beta shares 90% and 89% amino acid (aa) sequences identity with mouse and rat FSH beta, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets follitropin subunit beta (gene FSHB) in human and mouse samples and is validated for IHC and Western blot. The predicted molecular weight is 14.7 kDa, and the supplier reports an observed band at about 36 kDa.
FSHB is the hormone-specific β subunit of follicle-stimulating hormone (FSH). It pairs with the common glycoprotein α subunit (CGA) to form active FSH, which is released by the pituitary and acts through FSHR to support ovarian follicle growth and spermatogenesis. FSHβ is also studied in fish, as a model of reproductive endocrinology.
| Target | Follitropin subunit beta (gene FSHB; UniProt P01225, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 14.7 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, human mammry cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human FSH beta fragment (Asn19–Glu129), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 90% and 89%, respectively.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes follitropin subunit beta as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jia Yudong et al. (2023) Morphological comparison and gonadotropins cell localization of mature female turbot and mouse pituitary. Journal of Oceanology and Limnology. 10.1007/s00343-022-2250-7
Z. He et al. (2013) Long-Term Effects of Conversion From Cyclosporine to Rapamycin on Testicular Function and Morphology in a Rat Transplantation Model. Transplantation Proceedings. 10.1016/j.transproceed.2012.03.067
Yuxin Ma et al. (2011) Toxicological effects of acrylamide on the reproductive system of weaning male rats. Toxicology and Industrial Health. 10.1177/0748233710394235
