| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras GTPase-activating protein-binding protein 1; G3BP-1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1; G3BP1; G3BP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human G3BP/G3BP1, which shares 64.7% amino acid (aa) sequence identity with mouse G3BP1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ras GTPase-activating protein-binding protein 1 (gene G3BP1) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 52.2 kDa, and the supplier reports an observed band at about 68 kDa.
G3BP1 is an RNA-binding protein that is a core nucleator of stress granules, the membraneless mRNA–protein condensates that form when translation stalls under stress. It triggers their assembly through RNA-driven phase separation and also shows helicase activity in vitro. G3BP1 is widely used as a stress granule marker and is studied in stress responses, antiviral immunity and neurodegeneration.
| Target | Ras GTPase-activating protein-binding protein 1 (gene G3BP1; UniProt Q13283, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.2 kDa |
| Observed MW | 68 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human 293T cells, human A549 cells, human MDA-MB-453 cells, human U-87MG cells, human Caco-2 cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human adenocarcinoma of the right colon tissue, human cervical cancer tissue, human liver cancer tissue, human lymphoma tissue, human renal cells carcinoma tissue, human stomach cancer tissue, human tonsil tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Synthetic peptide from the middle region of human G3BP/G3BP1. Sequence identity with the mouse ortholog: 64.7%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ras GTPase-activating protein-binding protein 1 in the cytoplasm, perikaryon and nucleus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shun Lv et al. (2017) MicroRNA-129-5p inhibits 3T3-L1 preadipocyte proliferation by targeting G3BP1. Animal Cells and Systems. 10.1080/19768354.2017.1337046
