Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband®

Overview

This antibody is intended for detection of SLC6A1 (Long-chain-fatty-acid--CoA ligase 5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband® catalog # A05109. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A1, different from the related mouse and rat sequences by two amino acids.
• Molecular weight context: reported MW: 67 kDa; calculated MW: 67 kDa
• Reactivity: Human,Mouse,Rat
• Applications: IF, IHC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Long-chain-fatty-acid--CoA ligase 5; Sodium- and chloride-dependent GABA transporter 1. GABA transporter 1 (GAT1), also known as sodium- and chloride-dependent GABA transporter 1, is a protein that in humans is encoded by the SLC6A1 gene. GABA Transporter 1 uses Na+ and Cl- to create a gradient, which removes or adds GABA to extracellular spaces in the cerebrum and cerebellum. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl-: 1 GABA. The activity of GAT1 is largely dependent on the presence of Na+, while Cl- assists by increasing the ability for GAT-1 to uptake GABA. Functional note: Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. Reported localization: Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). . Expression/tissue context: Expressed in many tissues, highest levels in skeletal muscle.

Research relevance and current trends

• Neuroscience: Researchers commonly examine how SLC6A1 (Long-chain-fatty-acid--CoA ligase 5) relates to this theme using model systems and orthogonal readouts.
• Neurotransmitter: Researchers commonly examine how SLC6A1 (Long-chain-fatty-acid--CoA ligase 5) relates to this theme using model systems and orthogonal readouts.
• Transporters: Researchers commonly examine how SLC6A1 (Long-chain-fatty-acid--CoA ligase 5) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative SLC6A1 (Long-chain-fatty-acid--CoA ligase 5) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of SLC6A1 (Long-chain-fatty-acid--CoA ligase 5) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.