| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein; MM46; GABARAP; FLC3B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human GABARAP, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects gamma-aminobutyric acid receptor-associated protein (gene GABARAP) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 13.9 kDa, and the supplier reports an observed band at about 18 kDa.
GABARAP is a ubiquitin-like protein of the ATG8 family. It was first found to bind GABA(A) receptors and help their trafficking, and it is also conjugated to phosphatidylethanolamine on autophagic membranes, where GABARAP-subfamily proteins are important for late steps of autophagosome formation. GABARAP is studied in autophagy, mitophagy and neuronal receptor transport.
| Target | Gamma-aminobutyric acid receptor-associated protein (gene GABARAP; UniProt O95166, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.005 mg NaN3 |
| Calculated MW | 13.9 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
Samples with a confirmed band (WB): human HEK293 cells, human Jurkat cells, human A549 cells, human HeLa cells, human U87 cells, human HepG2 cells, human U937 cells, rat liver tissue, rat pancreas tissue, mouse liver tissue, mouse pancreas tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human esophageal squamous carcinoma tissue, human ovarian cancer tissue, human renal carcinomar tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells, U251 cells.
Immunogen
Synthetic peptide from the N-terminal region of human GABARAP. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places gamma-aminobutyric acid receptor-associated protein in the endomembrane system, cytoplasm, Golgi apparatus membrane and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liang-Hui Zhan et al. (2020) Soporific Effect of Modified Suanzaoren Decoction and Its Effects on the Expression of CCK-8 and Orexin-A. Evidence-based Complementary and Alternative Medicine. 10.1155/2020/6984087
Jin Wang et al. (2018) Effects of L-655,708 on expression changes of GABA, glutamate, and beta-endorphin induced by propofol anesthesia in rats. European Journal of Inflammation. 10.1177/2058739218796708
