Anti-Galactosidase alpha Rabbit Monoclonal Antibody

Overview

This product is an anti-GLA antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23G29; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Galactosidase alpha Rabbit Monoclonal Antibody catalog # M01135. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: GLA (Myocyte-specific enhancer factor 2C).
• Antibody format: Monoclonal; clone 23G29; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

GLA (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle- specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2. . Reported cellular localization context: Nucleus. Tissue expression notes (as provided): Expressed in brain and skeletal muscle. .

Research relevance and current trends

• Research context keywords from the source record include: Calcium Signaling,Calmodulin Pathway,Cardiogenesis,Cardiovascular,Developmental Families,Domain Families,Epigenetics and Nuclear Signaling,Hypertrophy,Mesenchymal Stem Cells,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction,Signaling Pathway,Stem Cells,Transcription,Transcription Factors,Transcription Factors/Regulators.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate GLA antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect GLA expression by Western blot in cell or tissue lysates, Detect GLA in FFPE tissue sections by immunohistochemistry, Localize GLA by immunofluorescence/immunocytochemistry in cultured cells, Quantify GLA-positive cells by flow cytometry in single-cell suspensions, Enrich GLA by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 46 kDa; calculated MW: 51221 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 46 kDa
• Cellular localization (provided): Nucleus.
• Tissue details (provided): Expressed in brain and skeletal muscle. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.