| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Galactoside 2-alpha-L-fucosyltransferase 1;2.4.1.69;Alpha (1,2)FT 1;Blood group H alpha 2-fucosyltransferase;Fucosyltransferase 1;GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1;FUT1;H, HSC; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FUT1, different from the related mouse and rat sequences by seven amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FUT1 (Galactoside 2-alpha-L-fucosyltransferase 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Galactoside 2-alpha-L-fucosyltransferase 1/FUT1 Antibody Picoband® catalog # PB9593. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human FUT1, different from the related mouse and rat sequences by seven amino acids.
- Molecular weight context: reported MW: 50 kDa; calculated MW: 41251 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Galactoside 2-alpha-L-fucosyltransferase 1; Galactoside 2-alpha-L-fucosyltransferase 1. Galactoside 2-alpha-L-fucosyltransferase 1 is an enzyme that in humans is encoded by the FUT1 gene. It is mapped to 19q13.3. The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the soluble A and B antigen synthesis pathway. This gene is one of two encoding the galactoside 2-L-fucosyltransferase enzyme. Mutations in this gene are a cause of the H-Bombay blood group. Functional note: Creates a soluble precursor oligosaccharide FuC-alpha ( (1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. Reported localization: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Membrane-bound form in trans cisternae of Golgi. Expression/tissue context: Ubiquitous.
Research relevance and current trends
- Golgi Proteins: Researchers commonly examine how FUT1 (Galactoside 2-alpha-L-fucosyltransferase 1) relates to this theme using model systems and orthogonal readouts.
- Protein Trafficking: Researchers commonly examine how FUT1 (Galactoside 2-alpha-L-fucosyltransferase 1) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how FUT1 (Galactoside 2-alpha-L-fucosyltransferase 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FUT1 (Galactoside 2-alpha-L-fucosyltransferase 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of FUT1 (Galactoside 2-alpha-L-fucosyltransferase 1) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the glycosyltransferase 11 family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
