| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | N-acetylgalactosamine-6-sulfatase;3.1.6.4;Chondroitinsulfatase;Chondroitinase;Galactose-6-sulfate sulfatase;GalN6S;N-acetylgalactosamine-6-sulfate sulfatase;GalNAc6S sulfatase;GALNS; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human GALNS recombinant protein (Position: Y181-N289). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GALNS (Peroxisomal multifunctional enzyme type 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GALNS Antibody Picoband® catalog # A02954-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human GALNS recombinant protein (Position: Y181-N289). (reported region: Y181-N289).
- Molecular weight context: reported MW: 58 kDa; calculated MW: 58026 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Peroxisomal multifunctional enzyme type 2; N-acetylgalactosamine-6-sulfatase. N-acetylgalactosamine-6-sulfatase is an enzyme that, in humans, is encoded by the GALNS gene. This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. Functional note: Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity. Reported localization: Lysosome. Expression/tissue context: Highly expressed in spleen, thymus, small intestine and peripheral blood leukocytes. Expressed in resting B- cells and activated T-cells, but not in resting T-cells.
Research relevance and current trends
- Cytoskeleton/ECM: Researchers commonly examine how GALNS (Peroxisomal multifunctional enzyme type 2) relates to this theme using model systems and orthogonal readouts.
- ECM Proteins: Researchers commonly examine how GALNS (Peroxisomal multifunctional enzyme type 2) relates to this theme using model systems and orthogonal readouts.
- Extracellular Matrix: Researchers commonly examine how GALNS (Peroxisomal multifunctional enzyme type 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GALNS (Peroxisomal multifunctional enzyme type 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of GALNS (Peroxisomal multifunctional enzyme type 2) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
