| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Protein F1;Gap43; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | GAP-43 from neonatal rat forebrain membranes. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GAP43 Antibody (Monoclonal, GAP-7B10) is an antibody targeting GAP43. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: GAP-7B10; isotype: Mouse IgG2a; reactivity: Human,Mouse,Rat; observed MW: 426 kDa; calculated MW: 23603 MW.
Boster Bio Anti-GAP43 Antibody (Monoclonal, GAP-7B10) catalog # MA1042. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: GAP43 — Neuromodulin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: GAP-7B10; Isotype: Mouse IgG2a
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 426 kDa; Calculated: 23603 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity). .
Scientific background (datasheet): GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.
Cellular localization (datasheet): Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes.
Sequence similarities (datasheet): Belongs to the neuromodulin family.
Research relevance and current trends
- Commonly studied in contexts related to Axonal Guidance Proteins,Cell Type Marker,Growth and Development,Neurology Process,Neuron Marker,Neuroscience,Synapse Marker.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
