| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | HRP-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Gene ID | |
| Host | |
| Immunogen | HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GAPDH Antibody Picoband® (HRP) is an antibody for GAPDH detection raised in Rabbit (Polyclonal, IgG, HRP), with reported reactivity: Chicken,Human,Monkey,Mouse,Rat,Zebrafish. Commonly used in WB workflows.
Key elements and design rationale
- Target: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase); UniProt: P04406; NCBI Gene: 2597
- Antibody format: Rabbit, Polyclonal, IgG, HRP
- Molecular weight: 36 kDa, calculated 36053 MW
- Applications: WB
Vendor description (summary): Boster Bio Anti-GAPDH Antibody (HRP) (Catalog # A00227-HRP).
Biological background
Biological context: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Expression and localization notes: cellular localization: Cytoplasm, cytosol, tissue context: Widely expressed, with the highest expression in lung, spleen and thymus..
Common research applications
- Western blotting (WB): Compare GAPDH levels across samples and conditions using appropriate loading and biological controls.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
- Cross reactivity: HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
- Cellular localization: Cytoplasm, cytosol
- Tissue details: Widely expressed, with the highest expression in lung, spleen and thymus.
- Sequence similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
- Research category: Signal Transduction,Neuroscience
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
