| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; GAPD |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects glyceraldehyde-3-phosphate dehydrogenase (gene GAPDH) in chicken, human, monkey, mouse, rat and zebrafish samples and is validated for Western blot. The predicted molecular weight is 36.1 kDa, and the supplier reports an observed band at about 36 kDa.
GAPDH catalyzes a central step of glycolysis, the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. Because it is abundant and broadly expressed, it is one of the most widely used loading controls for Western blot, although it also has non-glycolytic roles in the nucleus, in membrane trafficking and in translational control.
| Target | Glyceraldehyde-3-phosphate dehydrogenase (gene GAPDH; UniProt P04406, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Chicken, Human, Monkey, Mouse, Rat, Zebrafish |
| Form | Liquid |
| Formulation | 50% glycerol; 0.9% NaCl; 0.2% Na2HPO4 |
| Calculated MW | 36.1 kDa |
| Observed MW | 36 kDa |
| Storage | Store at 4 °C. |
Validated Applications
| Western blot | 0.02–0.1 µg/mL |
|---|
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human A562 cells, human MCF-7 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue, chicken heart tissue, chicken liver tissue, chicken brain tissue, monkey heart tissue, monkey lung tissue, monkey kidney tissue, monkey liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.1 µg/mL overnight at 4 °C, ECL detection.
Immunogen
HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
Reactivity Notes
The supplier lists reactivity with chicken, human, monkey, mouse, rat and zebrafish. UniProt places glyceraldehyde-3-phosphate dehydrogenase in the cytoplasm, nucleus and membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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