| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Intraflagellar transport protein 88 homolog; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10; TPR repeat protein 10; IFT88; TG737; TTC10 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GAS1 recombinant protein (Position: L40-D288). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GAS1 Antibody Picoband® is an antibody for GAS1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: GAS1 (intraflagellar transport 88); UniProt: P54826
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 40 kDa, calculated 104553 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-GAS1 Antibody Picoband® catalog # A06815-2.
Biological background
Biological context: Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
Expression and localization notes: cellular localization: Centriole. Cilium basal body. Centrosome. Cilium. Cytoplasm. Flagellum., tissue context: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas..
Common research applications
- Western blotting (WB): Compare GAS1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of GAS1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify GAS1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Growth arrest-specific protein 1 is a protein that in humans is encoded by the GAS1 gene. Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Centriole. Cilium basal body. Centrosome. Cilium. Cytoplasm. Flagellum.
- Tissue details: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
- Research category: Cell Adhesion Proteins,ECM Proteins,Neuroscience
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
