| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucin-6; MUC-6; Gastric mucin-6; MUC6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Gastric Mucin recombinant protein (Position: H52-R230). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mucin-6 (gene MUC6) in human and mouse samples and is validated for ELISA and Western blot. The predicted molecular weight is 257.1 kDa, and the supplier reports an observed band at about 257 kDa.
Mucin-6 (MUC6) is a gel-forming mucin produced by gastric mucous neck and antral gland cells and by the duodenal Brunner's glands. It helps form the protective mucus layer of the stomach, and it is used as a marker of gastric gland differentiation in pathology research.
| Target | Mucin-6 (gene MUC6; UniProt Q6W4X9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 257.1 kDa |
| Observed MW | 257 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse stomach tissue, human SGC-7901 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human Gastric Mucin fragment (His52–Arg230), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes mucin-6 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in the regenerative zone of gastric antrum, gastric body mucosa and gastric incisura mucosa. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
