| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Trans-acting T-cell-specific transcription factor GATA-3;GATA-binding factor 3;GATA3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GATA3 recombinant protein (Position: M1-E197). Human GATA3 shares 95% amino acid (aa) sequence identity with mouse GATA3. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GATA3 (Trans-acting T-cell-specific transcription factor GATA-3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GATA3 Antibody Picoband® catalog # PB9199. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human GATA3 recombinant protein (Position: M1-E197). Human GATA3 shares 95% amino acid (aa) sequence identity with mouse GATA3. (reported region: M1-E197).
- Molecular weight context: reported MW: 48 kDa; calculated MW: 47916 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Trans-acting T-cell-specific transcription factor GATA-3; Trans-acting T-cell-specific transcription factor GATA-3. Trans-acting T-cell-specific transcription factor GATA-3, also named HDR or GATA3, is a protein that in humans is encoded by the GATA3 gene. It is mapped to 10p14. This gene belongs to the GATA family of transcription factors. It regulates luminal epithelial cell differentiation in the mammary gland. The protein contains two GATA-type zinc fingers, it is an important regulator of T cell development and plays an important role in endothelial cell biology. GATA3 has been shown to promote the secretion of IL-4, IL-5, and IL-13 from Th2 cells, and induces the differentiation of Th0 cells towards this T cell subtype while suppressing their differentiation towards Th1 cells. Functional note: Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. . Reported localization: Nucleus. Expression/tissue context: T-cells and endothelial cells.
Research relevance and current trends
- Lineage Specification: Researchers commonly examine how GATA3 (Trans-acting T-cell-specific transcription factor GATA-3) relates to this theme using model systems and orthogonal readouts.
- Non-CD: Researchers commonly examine how GATA3 (Trans-acting T-cell-specific transcription factor GATA-3) relates to this theme using model systems and orthogonal readouts.
- Stem Cells: Researchers commonly examine how GATA3 (Trans-acting T-cell-specific transcription factor GATA-3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GATA3 (Trans-acting T-cell-specific transcription factor GATA-3) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Contains 2 GATA-type zinc fingers.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
