| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement component 1 Q subcomponent-binding protein, mitochondrial;ASF/SF2-associated protein p32;Glycoprotein gC1qBP;C1qBP;Hyaluronan-binding protein 1;Mitochondrial matrix protein p32;gC1q-R protein;p33;C1QBP;GC1QBP, HABP1, SF2P32; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human GC1q R recombinant protein (Position: E190-Q282). Human GC1q R shares 92.5% and 93.5% amino acid (aa) sequence identity with mouse and rat GC1q R, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets complement component 1 Q subcomponent-binding protein, mitochondrial (gene C1QBP) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 31.4 kDa, and the supplier reports an observed band at about 35 kDa.
C1QBP (also known as p32 or gC1qR) is a mainly mitochondrial protein originally identified by its binding to the globular heads of complement C1q. It supports mitochondrial protein synthesis and has been linked to ribosome biogenesis, apoptosis, splicing and inflammation, with a cell-surface pool also reported. Researchers study it in mitochondrial function, cancer metabolism and host–pathogen interactions.
| Target | Complement component 1 Q subcomponent-binding protein, mitochondrial (gene C1QBP; UniProt Q07021, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 31.4 kDa |
| Observed MW | 35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human SW620 cells, human 293T cells, human A431 cells, human HeLa- WT cells, human HeLa-C1QBP KO cells, rat brain tissue, rat spleen tissue, mouse brain tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, rat spleen tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human GC1q R fragment (Glu190–Gln282), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 92.5% and 93.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places complement component 1 Q subcomponent-binding protein, mitochondrial in the mitochondrion matrix, nucleus and cell membrane. Tissue expression noted by UniProt (human): Expressed on cell surface of peripheral blood cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Shuangping et al. (2025) Exploring the Role of Microglia Activation in Ischemia Stroke Based on the circDnajc1/miR-27a-5p/C1qc Signaling Axis. Molecular Neurobiology. 10.1007/s12035-025-05131-w
Yinghui Song et al. (2026) Localization of the Complement C1q-Binding Site on Echinococcus multilocularis Calreticulin Identified by Peptide Mapping. Tropical Medicine and Infectious Disease. 10.3390/tropicalmed11060146
