| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prolactin-inducible protein; Gross cystic disease fluid protein 15; GCDFP-15; Prolactin-induced protein; Secretory actin-binding protein; SABP; gp17; PIP; GCDFP15; GPIP4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GCDFP 15 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21P85 is a rabbit monoclonal antibody that recognizes prolactin-inducible protein (gene PIP) in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 16.6 kDa, and the supplier reports an observed band at about 17 kDa.
Prolactin-inducible protein (PIP, also called GCDFP-15) is a small secreted glycoprotein produced by apocrine and exocrine glands such as breast, salivary, lacrimal and sweat glands. Its expression is induced by prolactin and androgens, and it is widely used as a marker of apocrine and breast differentiation in tissue research.
| Target | Prolactin-inducible protein (gene PIP; UniProt P12273, human) |
|---|---|
| Clone | 21P85 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.6 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): T47D cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GCDFP 15.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes prolactin-inducible protein as a secreted protein. Tissue expression noted by UniProt (human): Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
