| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glial cell line-derived neurotrophic factor;hGDNF;Astrocyte-derived trophic factor;ATF;GDNF; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GDNF recombinant protein (Position: S78-I211). Human GDNF shares 93% amino acid (aa) sequence identity with both mouse and rat GDNF. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects glial cell line-derived neurotrophic factor (gene GDNF) in rat and human samples and is validated for Western blot. The predicted molecular weight is 23.7 kDa, and the supplier reports an observed band at about 24 kDa.
Glial cell line-derived neurotrophic factor (GDNF) is a secreted growth factor that strongly supports the survival of dopaminergic and motor neurons. It binds the coreceptor GFRα1 and signals through the RET tyrosine kinase. GDNF is also required for kidney development and spermatogonial stem cell maintenance, and it is studied in Parkinson's disease research.
| Target | Glial cell line-derived neurotrophic factor (gene GDNF; UniProt P39905, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 23.7 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat, Human) |
|---|
Samples with a confirmed band (WB): rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human GDNF fragment (Ser78–Ile211), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93%.
Reactivity Notes
The supplier lists reactivity with rat and human. UniProt describes glial cell line-derived neurotrophic factor as a secreted protein. Tissue expression noted by UniProt (human): In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ruisong Li et al. (2011) S100B Protein, Brain-Derived Neurotrophic Factor, and Glial Cell Line-Derived Neurotrophic Factor in Human Milk. PLoS One. 10.1371/journal.pone.0021663
Sun Zhaoxia et al. (2015) Prenatal nicotine exposure induced GDNF/c-Ret pathway repression-related fetal renal dysplasia and adult glomerulosclerosis in male offspring. Toxicology Research. 10.1039/c5tx00040h
Ying Ao et al. (2015) Low functional programming of renal AT2R mediates the developmental origin of glomerulosclerosis in adult offspring induced by prenatal caffeine exposure. Toxicology and Applied Pharmacology. 10.1016/j.taap.2015.05.007
Xiaoli Zhang et al. (2014) Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells. Reproductive Toxicology. 10.1016/j.reprotox.2014.01.009
Jie Qin et al. (2015) Concentrated growth factor increases Schwann cell proliferation and neurotrophic factor secretion and promotes functional nerve recovery in vivo. International Journal of Molecular Medicine. 10.3892/ijmm.2015.2438
A Lan et al. (2018) Surface functionalization of TiO2 nanotubes with minocycline and its in vitro biological effects on Schwann cells. Biomedical Engineering Online. 10.1186/s12938-018-0520-6
