| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | GDNF family receptor alpha-1;GDNF receptor alpha-1;GDNFR-alpha-1;GFR-alpha-1;RET ligand 1;TGF-beta-related neurotrophic factor receptor 1;GFRA1;GDNFRA, RETL1, TRNR1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GFRA1 (GDNF family receptor alpha-1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband® catalog # PB9202. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1. (reported region: D25-Q227).
- Molecular weight context: reported MW: 51 kDa; calculated MW: 51456 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
GDNF family receptor alpha-1; GDNF family receptor alpha-1. GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor or GFRA1, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to chromosome 10q25.3. The protein encoded by this gene is a member of the GDNF receptor family. GFRA1 is released by neuronal cells, Schwann cells, and injured sciatic nerve. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is also a candidate gene for Hirschsprung disease. Soluble GFRA1 mediates robust recruitment of RET to lipid rafts via a mechanism requiring the RET tyrosine kinase. Functional note: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity). . Reported localization: Cell membrane ; Lipid-anchor, GPI-anchor . Expression/tissue context: T-cells and endothelial cells.
Research relevance and current trends
- Growth and Development: Researchers commonly examine how GFRA1 (GDNF family receptor alpha-1) relates to this theme using model systems and orthogonal readouts.
- Neurology Process: Researchers commonly examine how GFRA1 (GDNF family receptor alpha-1) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how GFRA1 (GDNF family receptor alpha-1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GFRA1 (GDNF family receptor alpha-1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of GFRA1 (GDNF family receptor alpha-1) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the glutamate--cysteine ligase type 3 family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
