| Field | Specification |
|---|---|
| Target | |
| Alternative names | GDNF family receptor alpha-1;GDNF receptor alpha-1;GDNFR-alpha-1;GFR-alpha-1;RET ligand 1;TGF-beta-related neurotrophic factor receptor 1;GFRA1;GDNFRA, RETL1, TRNR1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes GDNF family receptor alpha-1 (gene GFRA1) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 51.5 kDa, and the supplier reports an observed band at about 51 kDa.
GFRα1 is a GPI-anchored cell surface coreceptor that binds GDNF with high affinity. The GDNF–GFRα1 complex recruits and activates the RET tyrosine kinase, supporting neuron survival, kidney development and spermatogonial stem cell maintenance. GFRα1 is studied in neuroscience and developmental biology.
| Target | GDNF family receptor alpha-1 (gene GFRA1; UniProt P56159, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 51.5 kDa |
| Observed MW | 51 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human GFRA1 fragment (Asp25–Gln227), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 97%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places GDNF family receptor alpha-1 in the cell membrane, Golgi apparatus and endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Lingling et al. (2022) Epigenetic Regulation of TET1-SP1 During Spermatogonia Self-Renewal and Proliferation. Frontiers in Physiology. 10.3389/fphys.2022.843825
Jiang Wu et al. (2025) Construction and Performance Evaluation of an Astaxanthin–Chitosan/Chitooligosaccharide Hydrogel System for Ex Vivo Culture of Murine Spermatogonial Stem Cells. Biology-Basel. 10.3390/biology14121664
Wang Jin et al. (2024) JMJD3 regulate H3K27me3 modification via interacting directly with TET1 to affect spermatogonia self-renewal and proliferation. BMC Genomics. 10.1186/s12864-024-10120-9
H. Cai et al. (2016) Enrichment and in vitro features of the putative gonocytes from cryopreserved testicular tissue of neonatal bulls. Andrology. 10.1111/andr.12229
Wen-Qian Zhu et al. (2021) Survivable potential of germ cells after trehalose cryopreservation of bovine testicular tissues. Cryobiology. 10.1016/j.cryobiol.2021.05.001
Yu Jiang et al. (2020) Cryopreservation of calf testicular tissues with knockout serum replacement. Cryobiology. 10.1016/j.cryobiol.2020.01.010
