| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Gelsolin;Actin-depolymerizing factor;ADF;Brevin;Gsn; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human plasma gelsolin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4) is an antibody targeting GSN. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: GS-2C4; isotype: Mouse IgG1; reactivity: Human,Rabbit; observed MW: 90 kDa; calculated MW: 86068 MW.
Boster Bio Anti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4) catalog # MA1044. Tested in IHC, WB applications. This antibody reacts with Human, Rabbit.
Key elements and design rationale
- Target: GSN — Gelsolin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: GS-2C4; Isotype: Mouse IgG1
- Species reactivity: Human,Rabbit
- Molecular weight guidance: Observed: 90 kDa; Calculated: 86068 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity). .
Scientific background (datasheet): Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.
Cellular localization (datasheet): Isoform 1: Secreted .
Tissue details (datasheet): GSN: Phagocytic cells, platelets, fibroblasts,nonmuscle cells, smooth and skeletal muscle cells.
Sequence similarities (datasheet): Belongs to the villin/gelsolin family.
Research relevance and current trends
- Commonly studied in contexts related to Actin Binding Proteins,Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
