| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Gelsolin;AGEL;Actin-depolymerizing factor;ADF;Brevin;GSN; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Gelsolin recombinant protein (Position: E580-A782). Human Gelsolin shares 94% and 95% amino acid (aa) sequences identity with mouse and rat Gelsolin, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GSN (Gelsolin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Gelsolin/GSN Antibody Picoband® catalog # PB9209. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Gelsolin recombinant protein (Position: E580-A782). Human Gelsolin shares 94% and 95% amino acid (aa) sequences identity with mouse and rat Gelsolin, respectively. (reported region: E580-A782).
- Molecular weight context: reported MW: 86 kDa; calculated MW: 85698 MW
- Reactivity: Human,Monkey,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Gelsolin; Gelsolin. Gelsolin, also known as GNS or brevin, is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. It is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol. Functional note: Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. . Reported localization: Isoform 2: Cytoplasm, cytoskeleton. Expression/tissue context: Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Research relevance and current trends
- Amino Acids: Researchers commonly examine how GSN (Gelsolin) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how GSN (Gelsolin) relates to this theme using model systems and orthogonal readouts.
- Neurotransmission: Researchers commonly examine how GSN (Gelsolin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GSN (Gelsolin) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of GSN (Gelsolin) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the villin/gelsolin family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
