| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glial fibrillary acidic protein;GFAP;Gfap; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | GFAP from pig spinal cord. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GFAP Antibody (Monoclonal, G-A-5) is an antibody targeting GFAP. Common applications include WB, IHC, IF, IHC-F. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: G-A-5; isotype: Mouse IgG1; reactivity: Human,Mouse,Pig,Rat; observed MW: 50 kDa; calculated MW: 49957 MW.
Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
Key elements and design rationale
- Target: GFAP — Glial fibrillary acidic protein
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: G-A-5; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Pig,Rat
- Molecular weight guidance: Observed: 50 kDa; Calculated: 49957 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Scientific background (datasheet): Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
Cellular localization (datasheet): Cytoplasm . Associated with intermediate filaments. .
Tissue details (datasheet): Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment. .
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Cell Adhesion Proteins,Cell Type Marker,Cell Type Markers,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Neural Stem Cells,Neuroscience,Signal Transduction,Stem Cells,Tags & Cell Markers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
