| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glial fibrillary acidic protein;GFAP;Gfap; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | GFAP from pig spinal cord. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone G-A-5 is a mouse monoclonal antibody that recognizes glial fibrillary acidic protein (gene Gfap) in human, mouse, pig and rat samples and is validated for Western blot, IHC and ICC/IF.
Glial fibrillary acidic protein (GFAP) is a type III intermediate filament protein expressed mainly in astrocytes. It is the classic marker for astrocytes and for reactive astrogliosis after injury or disease. Mutations in GFAP cause Alexander disease, and GFAP levels are studied as a marker of brain injury.
| Target | Glial fibrillary acidic protein (gene Gfap; UniProt P47819, rat) |
|---|---|
| Clone | G-A-5 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human, Mouse, Pig, Rat |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.5–1 µg/mL (Human, Mouse, Pig, Rat) |
|---|---|
| IHC (paraffin sections) | 0.4–1 µg/mL (Human, Pig, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Rat) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
GFAP from pig spinal cord.
Reactivity Notes
The supplier lists reactivity with human, mouse, pig and rat. UniProt places glial fibrillary acidic protein in the cytoplasm. Tissue expression noted by UniProt (rat): Expressed in the cortex and hippocampus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jun-Min Chen et al. (2025) 3-HKA Promotes Vascular Remodeling After Stroke by Modulating the Activation of A1/A2 Reactive Astrocytes. Advanced Science. 10.1002/advs.202412667
Bo Wu et al. (2025) Structure-Inspired Lineage-Specific Matrix for Endogenous Neurogenesis in Spinal Cord Injury. Research. 10.34133/research.0821
Li Kewei et al. (2023) Micro electrical fields induced MSC-sEVs attenuate neuronal cell apoptosis by activating autophagy via lncRNA MALAT1/miR-22-3p/SIRT1/AMPK axis in spinal cord injury. Journal of Nanobiotechnology. 10.1186/s12951-023-02217-2
Chen Lei et al. (2025) Cholecystokinin ameliorates cognitive impairment via inhibiting microglia phagocytosis of excitatory synapses in sepsis-associated encephalopathy mice. Journal of Neuroinflammation. 10.1186/s12974-025-03554-9
Xinjin Su et al. (2024) Chitosan-Modified Hydrogel Microsphere Encapsulating Zinc-Doped Bioactive Glasses for Spinal Cord Injury Repair by Suppressing Inflammation and Promoting Angiogenesis. Advanced Healthcare Materials. 10.1002/adhm.202402129
Chaohui Wang et al. (2026) Rhodiosin promotes cerebral angiogenesis in mice with ischemic stroke via PI3K/Akt pathway activation. Phytomedicine. 10.1016/j.phymed.2026.158247
Zhang Rongrong et al. (2018) RGMa mediates reactive astrogliosis and glial scar formation through TGFβ1/Smad2/3 signaling after stroke. Cell Death and Differentiation. 10.1038/s41418-018-0058-y
Kaili Yin et al. (2024) Tak1 licenses mitochondrial transfer from astrocytes to POMC neurons to maintain glucose and cholesterol homeostasis. Cell Reports. 10.1016/j.celrep.2024.114983
Song-Ji Li et al. (2023) CNTN1 Aggravates Neuroinflammation and Triggers Cognitive Deficits in Male Mice by Boosting Crosstalk between Microglia and Astrocytes. Aging and Disease. 10.14336/AD.2023.0228
Xin Liu et al. (2012) Protective effects of cationic bovine serum albumin-conjugated PEGylated tanshinone IIA nanoparticles on cerebral ischemia. Biomaterials. 10.1016/j.biomaterials.2012.10.017
