| Field | Specification |
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| Conjugate | |
| Formulation | |
| Host | |
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| UniProt # |
Metabolic Enzymes
Product Overview
Recombinant anti-GFRAL (GDNF Family Receptor alpha Like) monoclonal antibody which recognizes GFRAL. This recombinant antibody has been tested for specific binding to purified recombinant human GFRAL (BPS Bioscience #101013) in an ELISA binding assay.
| Clonality | Monoclonal |
|---|---|
| Host | Human |
| Antibody Type | IgG |
| Reactivity | Human |
| Purity | ≥90% |
| Molecular Weight | High Chain: 50 kDa; Low Chain: 24 kDa |
| Formulation | 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol |
| UniProt | Q6UXV0 |
| Storage | At least 12 months at -80°C |
Validated Applications
- Enzyme Activity
- Compound Profiling
Assay conditions: An ELISA binding assay was performed with this antibody and its target protein, GFRAL (BPS Bioscience #101013). The antibody was coated onto an assay plate overnight at 2 µg/ml at 4°. On the next day, the plate was washed on blocked. Then, serial dilutions of the target protein were added (0 to 200 nM) for 1 hour at room temperature. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Then we measured absorbance on a plate reader at 450 nm. EC50 = 2.4 nM
Usage note: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.
Immunogen / Antigen Information
Immunogen information not provided. Please refer to the product datasheet for detailed antigen information.
Cross-Reactivity & Specificity
Cross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.
Safety & Handling
Avoid freeze/thaw cycles.
For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.
Related Products
- 101012
- 101013
This antibody has been validated for: Enzyme Activity, Compound Profiling. Optimal dilutions and concentrations should be empirically determined for each specific application and sample type. Refer to the product datasheet for suggested starting conditions.
This antibody should be stored at the indicated temperature to maintain stability. Store at At least 12 months at -80°C. Avoid repeated freeze-thaw cycles, which can reduce antibody performance and increase aggregation. Centrifuge briefly after thawing and keep on ice during use.
Please refer to the BPS Bioscience datasheet for detailed immunogen information.
Cross-reactivity data is limited; refer to the product datasheet for available information.
BPS Bioscience offers multiple antibody formats for key checkpoint and immunotherapy targets. Contact BioHippo (support@biohippo.com) or click "Talk to a Scientist" to inquire about additional conjugate formats, bulk quantities, or custom formulations.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
