| Field | Specification |
|---|---|
| Target | |
| Alternative names | Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46; GHRL; MTLRP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Ghrelin/GHRL, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets appetite-regulating hormone (gene GHRL) in human, mouse and rat samples and is validated for IHC. The predicted molecular weight is 12.9 kDa.
The GHRL gene encodes a precursor peptide that is processed into ghrelin and obestatin. Ghrelin, mainly made in the stomach and activated by octanoyl modification, binds the receptor GHSR to trigger growth hormone release and stimulate appetite and gastric acid secretion. It is widely studied in feeding behavior, energy balance and metabolism.
| Target | Appetite-regulating hormone (gene GHRL; UniProt Q9UBU3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 12.9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with confirmed staining (IHC): human gastric cancer tissue, human stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human ghrelin/GHRL. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes appetite-regulating hormone as a secreted protein. Tissue expression noted by UniProt (human): Highest level in stomach.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
