| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transforming growth factor beta activator LRRC32; Garpin; Glycoprotein A repetitions predominant; GARP; Leucine-rich repeat-containing protein 32; LRRC32; D11S833E |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Giantin/GOLGB1 recombinant protein (Position: L153-E950). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Giantin/GOLGB1 Antibody Picoband® is an antibody for GOLGB1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: GOLGB1 (leucine rich repeat containing 32); UniProt: Q14789
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 376 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Giantin/GOLGB1 Antibody Picoband® catalog # A08226-1.
Biological background
Biological context: Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta. Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity).
Expression and localization notes: cellular localization: Cell membrane. Single-pass type I membrane protein. Cell surface., tissue context: Heart, placenta, skeletal muscle, kidney, lung and pancreas..
Common research applications
- Western blotting (WB): Compare GOLGB1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of GOLGB1 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify GOLGB1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Giantin or Golgin subfamily B member 1 is a protein that in humans is encoded by the GOLGB1 gene. GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family. GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton. It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis. GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome. Human GOLGB1 is 3259 amino acids (aa) in length, and over aa 1596-1702, shares 92% and 91% sequence identity with its mouse and rat orthologs, respectively.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Single-pass type I membrane protein. Cell surface.
- Tissue details: Heart, placenta, skeletal muscle, kidney, lung and pancreas.
- Research category: Immune System Diseases,Immunology,Protein Trafficking,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
