| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Gastric inhibitory polypeptide;GIP;Glucose-dependent insulinotropic polypeptide;Gip; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse GIP recombinant protein (Position: Y44-Q85). Mouse GIP shares 92.9% amino acid (aa) sequence identity with both human and rat GIP. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Gip (Gastric inhibitory polypeptide) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GIP Antibody Picoband® catalog # PB9947. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse GIP recombinant protein (Position: Y44-Q85). Mouse GIP shares 92.9% amino acid (aa) sequence identity with both human and rat GIP. (reported region: Y44-Q85).
- Molecular weight context: reported MW: 18 kDa; calculated MW: 16389 MW
- Reactivity: Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Gastric inhibitory polypeptide; Gastric inhibitory polypeptide. Gastric inhibitory polypeptide (GIP), also known as the glucose-dependent insulinotropic peptide, is an inhibiting hormone of the secretin family of hormones. GIP is thought to have significant effects on fatty acid metabolism through stimulation of lipoprotein lipase activity in adipocytes. Additionally, GIP release has been demonstrated in the ruminant animal and may play a role in nutrient partitioning in milk production (lipid metabolism). Recently, GIP appeared as a major player in bone remodelling. It was evidenced that genetic ablation of the GIP receptor in mice resulted in profound alterations of bone microarchitecture through modification of the adipokine network. Furthermore, the deficiency in GIP receptors has also been associated in mice with a dramatic decrease in bone quality and a subsequent increase in fracture risk. Functional note: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Reported localization: Secreted. Expression/tissue context: Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. .
Research relevance and current trends
- G Protein Signaling: Researchers commonly examine how Gip (Gastric inhibitory polypeptide) relates to this theme using model systems and orthogonal readouts.
- Growth Factors/Hormones: Researchers commonly examine how Gip (Gastric inhibitory polypeptide) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how Gip (Gastric inhibitory polypeptide) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Gip (Gastric inhibitory polypeptide) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Gip (Gastric inhibitory polypeptide) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
