| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gap junction beta-1 protein; Connexin-32; Cx32; GAP junction 28 kDa liver protein; GJB1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GJB1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26G06 is a rabbit monoclonal antibody that recognizes gap junction beta-1 protein (gene GJB1) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 32.0 kDa, and the supplier reports an observed band at about 32 kDa.
Connexin 32 (GJB1) is a gap junction protein found in liver, myelinating Schwann cells and other tissues. Its channels allow small molecules and ions to move between cells or between layers of the myelin sheath, and mutations in GJB1 cause X-linked Charcot-Marie-Tooth neuropathy.
| Target | Gap junction beta-1 protein (gene GJB1; UniProt P08034, human) |
|---|---|
| Clone | 26G06 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 32.0 kDa |
| Observed MW | 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GJB1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places gap junction beta-1 protein in the cell membrane and cell junction. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
