| Field | Specification |
|---|---|
| Target | |
| Alternative names | Golgi apparatus protein 1; CFR-1; Cysteine-rich fibroblast growth factor receptor; E-selectin ligand 1; ESL-1; Golgi sialoglycoprotein MG-160; GLG1; CFR1; ESL1; MG160 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GLG1 recombinant protein (Position: E173-K1040). Human GLG1 shares 98.5% and 98% amino acid (aa) sequence identity with mouse and rat GLG1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets golgi apparatus protein 1 (gene GLG1) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
Golgi apparatus protein 1 (GLG1), also called E-selectin ligand-1 or MG-160, is a glycoprotein located mainly in the Golgi. It binds fibroblast growth factors and, on leukocytes, serves as a ligand for E-selectin on endothelial cells, linking it to growth factor handling and leukocyte adhesion.
| Target | Golgi apparatus protein 1 (gene GLG1; UniProt Q92896, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human GLG1 fragment (Glu173–Lys1040), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98.5% and 98%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places golgi apparatus protein 1 in the Golgi apparatus membrane and Golgi outpost. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shasha Song et al. (2016) Hypoxia inhibits pulmonary artery endothelial cell apoptosis via the e-selectin/biliverdin reductase pathway. Microvascular Research. 10.1016/j.mvr.2016.03.009
