| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Zinc finger protein GLI1;Glioma-associated oncogene;Oncogene GLI;GLI1;GLI; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Gli1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Gli1 Rabbit Monoclonal Antibody is an antibody targeting GLI1. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: GCG-7; isotype: Rabbit IgG; reactivity: Human; observed MW: 40 kDa; calculated MW: 117904 MW.
Boster Bio Anti-Gli1 Rabbit Monoclonal Antibody catalog # M00527. Tested in WB application. This antibody reacts with Human.
Key elements and design rationale
- Target: GLI1 — Zinc finger protein GLI1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: GCG-7; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 40 kDa; Calculated: 117904 MW
Biological background
Protein function (datasheet): Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. .
Cellular localization (datasheet): Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
Tissue details (datasheet): Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. .
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Domain Families,Epigenetics and Nuclear Signaling,Krueppel Like Factor,Neural Signal Transduction,Neurology Process,Neuroscience,Nuclear,Oncoproteins/Suppressors,Signaling Pathways,Stem Cells,Transcription,Tumor Suppressors,Zinc Finger.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
