| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glucagon;Glicentin;Glicentin-related polypeptide;GRPP;Oxyntomodulin;OXM;OXY;Glucagon;Glucagon-like peptide 1;GLP-1;Incretin hormone;Glucagon-like peptide 1 (7-37);GLP-1 (7-37);Glucagon-like peptide 1 (7-36);GLP-1 (7-36);Glucagon-like peptide 2;GLP-2;GCG; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Glucagon/GCG, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects pro-glucagon (gene GCG) in human, mouse and rat samples and is validated for ICC/IF and IHC. The predicted molecular weight is 20.9 kDa.
Proglucagon (GCG) is processed into several hormones depending on the tissue. In pancreatic α cells it yields glucagon, which raises blood glucose by stimulating hepatic glucose output, and in intestinal L cells it yields GLP-1 and GLP-2, which boost insulin secretion and support gut growth. GCG products are central to studies of glucose homeostasis, diabetes and obesity.
| Target | Pro-glucagon (gene GCG; UniProt P01275, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 20.9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Rat) |
Samples with confirmed staining (IHC): mouse pancreas tissue, rat pancreas tissue, human pancreatic cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): mouse pancreas tissue, rat pancreas tissue.
Immunogen
Synthetic peptide from the N-terminal region of human glucagon/GCG. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes pro-glucagon as a secreted protein. Tissue expression noted by UniProt (human): [Glucagon]: Secreted in the A cells of the islets of Langerhans...
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Jitai et al. (2019) Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure. Cell Death & Disease. 10.1038/s41419-019-1552-y
Ting Liu et al. (2024) Mannogalactoglucan from mushrooms protects pancreatic islets via restoring UPR and promotes insulin secretion in T1DM mice. Food Science and Human Wellness. 10.26599/FSHW.2022.9250117
Zhang Yong et al. (2016) The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlying signaling mechanisms in a rat model of diabetes mellitus. Scientific Reports. 10.1038/srep34284
Jingjing Yang et al. (2024) Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR-19b-3p/SOCS3/STAT3 axis mediated autophagy. IUBMB Life. 10.1002/iub.2907
Li-hui Gao et al. (2013) A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice. Journal of Ethnopharmacology. 10.1016/j.jep.2013.11.024
Wu Xinmou et al. (2017) Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro. Environmental Science and Pollution Research. 10.1007/s11356-017-0072-5
Chen Lu-Lu et al. (2010) Effect of catch-up growth after food restriction on the entero-insular axis in rats. Nutrition & Metabolism. 10.1186/1743-7075-7-45
Mengshi Li et al. (2018) Generation and Biological Characteristics of a Mouse Model of Breast Cancer that Copresents with Diabetes Mellitus. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. 10.1002/ar.23945
