| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Solute carrier family 2, facilitated glucose transporter member 5;Fructose transporter;Glucose transporter type 5, small intestine;GLUT-5;SLC2A5;GLUT5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SLC2A5, different from the related mouse and rat sequences by nine amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SLC2A5 (Solute carrier family 2, facilitated glucose transporter member 5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband® catalog # PB9960. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human SLC2A5, different from the related mouse and rat sequences by nine amino acids.
- Molecular weight context: reported MW: 55 kDa; calculated MW: 54974 MW
- Reactivity: Human,Rat
- Applications: Flow Cytometry, IF, IHC, IHC-F, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Solute carrier family 2, facilitated glucose transporter member 5; Solute carrier family 2, facilitated glucose transporter member 5. SLC2A5, also known as GLUT5 (Glucose transporter 5), is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. Functional note: Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter. Reported localization: Apical cell membrane ; Multi- pass membrane protein . Membrane; Multi-pass membrane protein. Localized on the apical membrane of the small intestine and the proximal tubule of the kidney. . Expression/tissue context: Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.
Research relevance and current trends
- Carbohydrate Metabolism: Researchers commonly examine how SLC2A5 (Solute carrier family 2, facilitated glucose transporter member 5) relates to this theme using model systems and orthogonal readouts.
- Channels: Researchers commonly examine how SLC2A5 (Solute carrier family 2, facilitated glucose transporter member 5) relates to this theme using model systems and orthogonal readouts.
- Energy Metabolism: Researchers commonly examine how SLC2A5 (Solute carrier family 2, facilitated glucose transporter member 5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SLC2A5 (Solute carrier family 2, facilitated glucose transporter member 5) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of SLC2A5 (Solute carrier family 2, facilitated glucose transporter member 5) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
