| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Solute carrier family 2, facilitated glucose transporter member 9; Glucose transporter type 9; GLUT-9; Urate transporter; SLC2A9; GLUT9 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human GLUT9/SLC2A9 recombinant protein (Position: R7-P540). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GLUT9/SLC2A9 Antibody Picoband® is an antibody for SLC2A9 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SLC2A9 (solute carrier family 2 (facilitated glucose transporter), member 9); UniProt: Q9NRM0; NCBI Gene: 56606
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 59 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-GLUT9/SLC2A9 Antibody Picoband® catalog # A02245.
Biological background
Biological context: Urate transporter, which may play a role in the urate reabsorption by proximal tubules. Does not transport glucose, fructose or galactose.
Expression and localization notes: cellular localization: Basolateral cell membrane. Multi-pass membrane protein. Plasma membrane. Apical cell membrane. Multi-pass membrane protein. Basolateral cell membrane. Multi-pass membrane protein., tissue context: Most strongly expressed in basolateral membranes of proximal renal tubular cells, liver and placenta. Also detected in lung, blood leukocytes, heart skeletal muscle and chondrocytes from articular cartilage. Isoform 2 is only detected in the apical membranes of polarized renal tubular cells and placenta. Isoform 1 and isoform 2 are detected in kidney membrane (at protein level)..
Common research applications
- Western blotting (WB): Compare SLC2A9 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SLC2A9 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify SLC2A9-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Solute carrier family 2, facilitated glucose transporter member 9, also known as SLC2A9, is a protein that in humans is encoded by the SLC2A9 gene. This gene encodes a member of the SLC2A facilitative glucose transporter family. Members of this family play a significant role in maintaining glucose homeostasis. This gene is mapped to 4p16.1. The encoded protein may play a role in the development and survival of chondrocytes in cartilage matrices. This gene transports urate and fructose. It may have a role in the urate reabsorption by proximal tubules. This gene also transports glucose at low rate.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Basolateral cell membrane. Multi-pass membrane protein. Plasma membrane. Apical cell membrane. Multi-pass membrane protein. Basolateral cell membrane. Multi-pass membrane protein.
- Tissue details: Most strongly expressed in basolateral membranes of proximal renal tubular cells, liver and placenta. Also detected in lung, blood leukocytes, heart skeletal muscle and chondrocytes from articular cartilage. Isoform 2 is only detected in the apical membranes of polarized renal tubular cells and placenta. Isoform 1 and isoform 2 are detected in kidney membrane (at protein level).
- Research category: Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
