| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glutamate receptor 3;GluR-3;AMPA-selective glutamate receptor 3;GluR-C;GluR-K3;Glutamate receptor ionotropic, AMPA 3;GluA3;GRIA3;GLUR3, GLURC; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GRIA3 recombinant protein (Position: G29-M360). Human GRIA3 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA3. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GRIA3 (Glutamate receptor 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Glutamate receptor 3/GRIA3 Antibody Picoband® catalog # PB9206. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human GRIA3 recombinant protein (Position: G29-M360). Human GRIA3 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA3. (reported region: G29-M360).
- Molecular weight context: reported MW: 101 kDa; calculated MW: 101157 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Glutamate receptor 3; Glutamate receptor 3. Glutamate receptor 3 is a protein that in humans is encoded by the GRIA3 gene. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. It is mapped to Xq25. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. Functional note: Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. . Reported localization: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Interaction with CNIH2 and CNIH3 promotes cell surface expression. . Expression/tissue context: Widely expressed in brain.
Research relevance and current trends
- Amino Acids: Researchers commonly examine how GRIA3 (Glutamate receptor 3) relates to this theme using model systems and orthogonal readouts.
- G Protein Signaling: Researchers commonly examine how GRIA3 (Glutamate receptor 3) relates to this theme using model systems and orthogonal readouts.
- Ligand-Gated Ion Channels: Researchers commonly examine how GRIA3 (Glutamate receptor 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GRIA3 (Glutamate receptor 3) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
