| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phospholipid hydroperoxide glutathione peroxidase GPX4; PHGPx; Glutathione peroxidase 4; GPx-4; GSHPx-4; GPX4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects phospholipid hydroperoxide glutathione peroxidase GPX4 in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 22.2 kDa, and the supplier reports an observed band at about 19 kDa.
Glutathione peroxidase 4 (GPX4) is a selenoprotein that reduces phospholipid hydroperoxides directly within membranes. By preventing lipid peroxidation, it protects cells from ferroptosis, an iron-dependent form of cell death, making GPX4 a central target in ferroptosis, neurodegeneration and cancer research.
| Target | Phospholipid hydroperoxide glutathione peroxidase GPX4 (gene GPX4; UniProt P36969, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 22.2 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Samples with a confirmed band (WB): human HepG2 cells, human MCF-7 cells, human RT4 cells, monkey COS-7 cells, rat kidney tissue, rat testis tissue, mouse kidney tissue, human HCT116- WT cells, human HCT116-GPX4 KO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human testis tissue, mouse testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Glutathione Peroxidase 4/GPX4 fragment (Ala30–Phe197), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. Tissue expression noted by UniProt (human): Present primarily in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiangping Wu et al. (2026) A polysaccharide from Pueraria lobata ameliorates hepatic fibrosis via gut microbiota-dependent suppression of ferroptosis. Phytomedicine. 10.1016/j.phymed.2026.158123
Xiao Ji et al. (2021) Maresin conjugates in tissue regeneration-1 suppresses ferroptosis in septic acute kidney injury. Cell and Bioscience. 10.1186/s13578-021-00734-x
Chuan-zhi Zhao et al. (2024) Exploring the mechanism of Ling-Gui-Zhu-Gan decoction in metabolic cardiomyopathy via inhibiting ferroptosis. Phytomedicine. 10.1016/j.phymed.2024.156234
Jiao Wang et al. (2026) SGLT2 inhibitors ameliorate myocardial hypertrophy in rats via AMPK/Nrf2-mediated ferroptosis suppression and antioxidant response. European Journal of Pharmacology. 10.1016/j.ejphar.2026.179049
Zhiguang Huang et al. (2025) Sinapic acid and glabridin synergistically mitigate androgenetic alopecia by modulating ferroptosis through stabilization of β-catenin against ubiquitin-dependent degradation. Biochemical Pharmacology. 10.1016/j.bcp.2025.117171
Jiani Zhan et al. (2025) IDO1-mediated AhR activation up-regulates pentose phosphate pathway via NRF2 to inhibit ferroptosis in lung cancer. Biochemical Pharmacology. 10.1016/j.bcp.2025.116913
Zhiwei Weng et al. (2023) Shikonin promotes ferroptosis in HaCaT cells through Nrf2 and alleviates imiquimod-induced psoriasis in mice. Chemico-Biological Interactions. 10.1016/j.cbi.2023.110788
Tao Xu et al. (2024) MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import. International Journal of Molecular Sciences. 10.3390/ijms251910832
Sihan Feng et al. (2025) Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study. Pharmaceuticals. 10.3390/ph18030345
Tiantian Guo et al. (2024) New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress. Poultry Science. 10.1016/j.psj.2024.103674
