| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phospholipid hydroperoxide glutathione peroxidase GPX4; PHGPx; Glutathione peroxidase 4; GPx-4; GSHPx-4; GPX4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 6I4E7 is a mouse monoclonal antibody that recognizes phospholipid hydroperoxide glutathione peroxidase GPX4 in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 22.2 kDa, and the supplier reports an observed band at about 19 kDa.
Glutathione peroxidase 4 (GPX4) is a selenoprotein that reduces phospholipid hydroperoxides directly within membranes. By preventing lipid peroxidation, it protects cells from ferroptosis, an iron-dependent form of cell death, making GPX4 a central target in ferroptosis, neurodegeneration and cancer research.
| Target | Phospholipid hydroperoxide glutathione peroxidase GPX4 (gene GPX4; UniProt P36969, human) |
|---|---|
| Clone | 6I4E7 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 22.2 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human Caco-2 cells, human 293T cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Recombinant human Glutathione Peroxidase 4/GPX4 fragment (Ala30–Phe197), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Present primarily in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Chao Liu et al. (2024) Contribution of impaired autophagy, mitochondrial dysfunction and abnormal lipolysis to epididymal aging in mice. Experimental Gerontology. 10.1016/j.exger.2024.112528
ShengJie Dai et al. (2024) Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma. Arab Journal of Gastroenterology. 10.1016/j.ajg.2024.07.006
