| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glutathione reductase, mitochondrial; GR; GRase; GSR; GLUR; GRD1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human Glutathione Reductase recombinant protein (Position: K256-R522). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GSR (Heterogeneous nuclear ribonucleoprotein A1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Glutathione Reductase/GSR Antibody Picoband® catalog # A01479-1. Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human Glutathione Reductase recombinant protein (Position: K256-R522). (reported region: K256-R522).
- Molecular weight context: reported MW: 52 kDa; calculated MW: 38747 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, Flow Cytometry, IF, IHC-P, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Heterogeneous nuclear ribonucleoprotein A1; glutathione-disulfide reductase. Glutathione reductase (GR), also known as glutathione-disulfide reductase (GSR), is an enzyme that in humans is encoded by the GSR gene. This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Functional note: Maintains high levels of reduced glutathione in the cytosol. Reported localization: Isoform Mitochondrial: Mitochondrion. Expression/tissue context: Expressed in peripheral blood leukocytes.
Research relevance and current trends
- Cancer: Researchers commonly examine how GSR (Heterogeneous nuclear ribonucleoprotein A1) relates to this theme using model systems and orthogonal readouts.
- Metabolism: Researchers commonly examine how GSR (Heterogeneous nuclear ribonucleoprotein A1) relates to this theme using model systems and orthogonal readouts.
- Mitochondrial: Researchers commonly examine how GSR (Heterogeneous nuclear ribonucleoprotein A1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GSR (Heterogeneous nuclear ribonucleoprotein A1) levels across conditions; band patterns may reflect isoforms and processing.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
