| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanine nucleotide-binding protein G; I; /G; S; T; subunit beta-2; G protein subunit beta-2; Transducin beta chain 2; GNB2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GNB2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21G58 is a rabbit monoclonal antibody that recognizes guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (gene GNB2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 37.3 kDa, and the supplier reports an observed band at about 35 kDa.
Gβ2 (GNB2) is one of the beta subunits of heterotrimeric G proteins. It pairs with a Gγ subunit to form a stable Gβγ dimer that supports nucleotide exchange on the Gα subunit and couples receptors to downstream effectors, so it is studied in G protein-coupled receptor signaling.
| Target | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (gene GNB2; UniProt P62879, human) |
|---|---|
| Clone | 21G58 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 37.3 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human MCF-7 cells, human SIHA cells, rat lung tissue, mouse small intestine tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human GNB2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expressed in all cardiac subcompartments and in the brain, with highest levels in the atrioventricular node and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
