| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Golgi phosphoprotein 3; Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS; GOLPH3; GPP34 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human GOLPH3 recombinant protein (Position: K56-K298). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GOLPH3 (Protein NDRG1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GOLPH3 Antibody Picoband® catalog # A01333-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human GOLPH3 recombinant protein (Position: K56-K298). (reported region: K56-K298).
- Molecular weight context: reported MW: 36 kDa; calculated MW: 79919 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Protein NDRG1; golgi phosphoprotein 3. Golgi phosphoprotein 3 (GOLPH3) is a human protein encoded by GOLPH3 gene. It is mapped to 5p13.3. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. Functional note: Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could inly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an in role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis. Reported localization: Golgi apparatus, Golgi stack membrane; Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes. . Expression/tissue context: Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Research relevance and current trends
- Subcellular Markers: Researchers commonly examine how GOLPH3 (Protein NDRG1) relates to this theme using model systems and orthogonal readouts.
- Tags & Cell Markers: Researchers commonly examine how GOLPH3 (Protein NDRG1) relates to this theme using model systems and orthogonal readouts.
- Vesicle Transport: Researchers commonly examine how GOLPH3 (Protein NDRG1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GOLPH3 (Protein NDRG1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of GOLPH3 (Protein NDRG1) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
