| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L; GPD1L; KIAA0089 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GPD1L recombinant protein (Position: A19-T351). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GPD1L Antibody Picoband® is an antibody for GPD1L detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: GPD1L (glycerol-3-phosphate dehydrogenase 1 like); UniProt: Q8N335
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 35-40 kDa, calculated 38 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-GPD1L Antibody Picoband® catalog # A07912-1.
Biological background
Biological context: Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD (H) balance induced by DPD1L.
Expression and localization notes: cellular localization: Cytoplasm., tissue context: Most highly expressed in heart tissue, with lower levels in the skeletal muscle, kidney, lung and other organs..
Common research applications
- Western blotting (WB): Compare GPD1L levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of GPD1L in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify GPD1L-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: GPD1L is a human gene. It is mapped to 3p22.3. The protein encoded by this gene contains a glycerol-3-phosphate dehydrogenase (NAD+) motif and shares 72% sequence identity with GPD1. The encoded protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). Defects in this gene are a cause of Brugada syndrome type 2 (BRS2) as well as sudden infant death syndrome (SIDS).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm.
- Tissue details: Most highly expressed in heart tissue, with lower levels in the skeletal muscle, kidney, lung and other organs.
- Research category: Cancer,Cell Biology,Energy Metabolism,Energy Transfer Pathways,Metabolic Signaling Pathways,Metabolism,Oxidative Stress,Pathways and Processes,Redox Metabolism,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
