| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L; GPD1L; KIAA0089 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GPD1L recombinant protein (Position: A19-T351). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes glycerol-3-phosphate dehydrogenase 1-like protein (gene GPD1L) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 38.4 kDa, and the supplier reports an observed band at about 35–40 kDa.
GPD1L is a cytosolic protein related to glycerol-3-phosphate dehydrogenase that is expressed in heart. It influences the cardiac sodium channel SCN5A, and GPD1L variants that reduce sodium current are linked to Brugada syndrome, so it is studied in cardiac electrophysiology.
| Target | Glycerol-3-phosphate dehydrogenase 1-like protein (gene GPD1L; UniProt Q8N335, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 38.4 kDa |
| Observed MW | 35–40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human PC-3 cells, human U87 cells, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human GPD1L fragment (Ala19–Thr351), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places glycerol-3-phosphate dehydrogenase 1-like protein in the cytoplasm. Tissue expression noted by UniProt (human): Most highly expressed in heart tissue, with lower levels in the skeletal muscle, kidney, lung and other organs. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
