| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transmembrane glycoprotein NMB; DC-HIL; Dendritic cell-associated transmembrane protein; Osteoactivin; Gpnmb; Dchil; Hgfin;;Nmb |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GPNMB/Gpnmb Picoband® Antibody is an antibody for Gpnmb detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Gpnmb (glycoprotein (transmembrane) nmb); UniProt: Q99P91; NCBI Gene: 93695
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 64 kDa, calculated 38779 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-GPNMB/Gpnmb Picoband® Antibody catalog # A02439-1.
Biological background
Biological context: Could be a melanogenic enzyme.
Expression and localization notes: cellular localization: Cell membrane. Single-pass type I membrane protein., tissue context: Monocyte/macrophage specific..
Common research applications
- Western blotting (WB): Compare Gpnmb levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Gpnmb in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify Gpnmb-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3; 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Single-pass type I membrane protein.
- Tissue details: Monocyte/macrophage specific.
- Research category: Immunoglobulins,Immunology,Innate Immunity
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
