| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transmembrane glycoprotein NMB; DC-HIL; Dendritic cell-associated transmembrane protein; Osteoactivin; Gpnmb; Dchil; Hgfin;;Nmb |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transmembrane glycoprotein NMB (gene Gpnmb) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 63.7 kDa, and the supplier reports an observed band at about 64 kDa.
GPNMB (glycoprotein nonmetastatic melanoma protein B, also called osteoactivin) is a type I transmembrane glycoprotein expressed in melanocytes, macrophages, osteoclasts and other cells. It has been linked to melanosome formation, bone remodeling and inflammation, and it is highly expressed in several cancers, so it is studied in tumor biology and macrophage function.
| Target | Transmembrane glycoprotein NMB (gene Gpnmb; UniProt Q99P91, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 63.7 kDa |
| Observed MW | 64 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer Heat mediated antigen retrieval was performed in EDTA buffer (pH80, epitope retrieval solution) The tissue, mouse brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): U87 cells, HEPA1–6 cells.
Immunogen
Recombinant mouse GPNMB/Gpnmb fragment (Arg164–Asp564), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transmembrane glycoprotein NMB in the cell membrane, melanosome membrane and early endosome membrane. Tissue expression noted by UniProt (mouse): May be up-regulated in bone metastatic breast cancer cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mingda Zhao et al. (2023) Bioabsorbable nano-micelle hybridized hydrogel scaffold prevents postoperative melanoma recurrence. Journal of Controlled Release. 10.1016/j.jconrel.2023.03.005
Qin Tingyu et al. (2022) Downregulation of glycoprotein non-metastatic melanoma protein B prevents high glucose-induced angiogenesis in diabetic retinopathy. Molecular and Cellular Biochemistry. 10.1007/s11010-022-04537-7
