| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glutamate receptor ionotropic, kainate 1;GluK1;Excitatory amino acid receptor 3;EAA3;Glutamate receptor 5;GluR-5;GluR5;GRIK1;GLUR5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GRIK1 recombinant protein (Position: R271-I450). Human GRIK1 shares 88% and 94% amino acid (aa) sequences identity with mouse and rat GRIK1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GRIK1 (Glutamate receptor ionotropic, kainate 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GRIK1 Antibody Picoband® catalog # PB9208. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human GRIK1 recombinant protein (Position: R271-I450). Human GRIK1 shares 88% and 94% amino acid (aa) sequences identity with mouse and rat GRIK1, respectively. (reported region: R271-I450).
- Molecular weight context: reported MW: 104 kDa; calculated MW: 103981 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Glutamate receptor ionotropic, kainate 1; Glutamate receptor ionotropic, kainate 1. Glutamate receptor, ionotropic, kainate 1, also known as GLUR5, is a protein that in humans is encoded by the GRIK1 gene. It is mapped to 21q21.3. This gene encodes one of the many ionotropic glutamate receptor (GluR) subunits that function as a ligand-gated ion channel. The specific GluR subunit encoded by this gene is of the kainate receptor subtype. Receptor assembly and intracellular trafficking of ionotropic glutamate receptors are regulated by RNA editing and alternative splicing. These receptors mediate excitatory neurotransmission and are critical for normal synaptic function. Exons of this gene are interspersed with exons from the C21or f41 gene, which is transcribed in the same orientation as this gene but does not seem to encode a protein. Functional note: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Reported localization: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Expression/tissue context: Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus.
Research relevance and current trends
- Amino Acids: Researchers commonly examine how GRIK1 (Glutamate receptor ionotropic, kainate 1) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how GRIK1 (Glutamate receptor ionotropic, kainate 1) relates to this theme using model systems and orthogonal readouts.
- Neurotransmission: Researchers commonly examine how GRIK1 (Glutamate receptor ionotropic, kainate 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GRIK1 (Glutamate receptor ionotropic, kainate 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of GRIK1 (Glutamate receptor ionotropic, kainate 1) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
