| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Beta-adrenergic receptor kinase 2;Beta-ARK-2;2.7.11.15;G-protein-coupled receptor kinase 3;ADRBK2;BARK2, GRK3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GRK3, different from the related mouse and rat sequences by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ADRBK2 (Beta-adrenergic receptor kinase 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GRK3/ADRBK2 Antibody Picoband® catalog # PB9682. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human GRK3, different from the related mouse and rat sequences by five amino acids.
- Molecular weight context: reported MW: 80 kDa; calculated MW: 79710 MW
- Reactivity: Human
- Applications: Flow Cytometry, IF, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Beta-adrenergic receptor kinase 2; Beta-adrenergic receptor kinase 2. Beta-adrenergic receptor kinase 2 (beta-ARK-2), also known as G-protein-coupled receptor kinase 3 (GRK3), is an enzyme that in humans is encoded by the ADRBK2 gene. The human ADRBK2 gene is located on 22q11. The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. Functional note: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. Reported localization: Secreted. Expression/tissue context: Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
Research relevance and current trends
- Ser/Thr Kinases: Researchers commonly examine how ADRBK2 (Beta-adrenergic receptor kinase 2) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how ADRBK2 (Beta-adrenergic receptor kinase 2) relates to this theme using model systems and orthogonal readouts.
- Signaling Pathway: Researchers commonly examine how ADRBK2 (Beta-adrenergic receptor kinase 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ADRBK2 (Beta-adrenergic receptor kinase 2) levels across conditions; band patterns may reflect isoforms and processing.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
