| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-X-C motif chemokine 3;Dendritic cell inflammatory protein 1 ;Cxcl3 ;Dcip1 , Gm1960 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse GRO gamma recombinant protein (Position: S32-S100). Mouse GRO gamma shares 60.6% and 90.6% amino acid (aa) sequence identity with human and rat GRO gamma, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CXCL3 (C-X-C motif chemokine 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GRO gamma/CXCL3 Antibody Picoband® catalog # PB9941. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse GRO gamma recombinant protein (Position: S32-S100). Mouse GRO gamma shares 60.6% and 90.6% amino acid (aa) sequence identity with human and rat GRO gamma, respectively. (reported region: S32-S100).
- Molecular weight context: reported MW: 25 kDa; calculated MW: 10686 MW
- Reactivity: Mouse
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-X-C motif chemokine 3; C-X-C motif chemokine 3. Chemokine (C-X-C motif) ligand 3 (CXCL3), also known as GRO protein gamma (GROg) and macrophage inflammatory protein-2-beta (MIP2b), is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Functional note: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. . Reported localization: Secreted . Expression/tissue context: Expressed in eye lens. .
Research relevance and current trends
- Chemokines: Researchers commonly examine how CXCL3 (C-X-C motif chemokine 3) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how CXCL3 (C-X-C motif chemokine 3) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how CXCL3 (C-X-C motif chemokine 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CXCL3 (C-X-C motif chemokine 3) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
