| Field | Specification |
|---|---|
| Target | |
| Alternative names | 78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP;HSPA5;GRP78; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects endoplasmic reticulum chaperone BiP (gene HSPA5) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 72.3 kDa, and the supplier reports an observed band at about 78 kDa.
BiP (HSPA5, also called GRP78) is the main HSP70 chaperone of the endoplasmic reticulum lumen. It helps fold and assemble newly made secreted and membrane proteins, holds misfolded ones for degradation, and controls the unfolded protein response sensors IRE1, PERK and ATF6. It is a standard marker of ER stress.
| Target | Endoplasmic reticulum chaperone BiP (gene HSPA5; UniProt P11021, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 72.3 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human U251 cells, human HepG2 cells, human K562 cells, human A431 cells, rat testis tissue, rat liver tissue, mouse testis tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human cerebral cortex tissue, mouse testis tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide from the C-terminal region of human GRP78 BiP. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endoplasmic reticulum chaperone BiP in the endoplasmic reticulum lumen, melanosome, cytoplasm and cell surface. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cixia Li et al. (2023) Ketogenic diet attenuates cognitive dysfunctions induced by hypoglycemia via inhibiting endoplasmic reticulum stress-dependent pathways. Food & Function. 10.1039/D3FO04007K
Wang Jingyu et al. (2018) Local Delivery of β-Elemene Improves Locomotor Functional Recovery by Alleviating Endoplasmic Reticulum Stress and Reducing Neuronal Apoptosis in Rats with Spinal Cord Injury. Cellular Physiology and Biochemistry. 10.1159/000492996
Yuzhen Sui et al. (2024) BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress. CNS Neuroscience & Therapeutics. 10.1111/cns.14840
Liuxin Wang et al. (2025) Cadmium-Induced Kidney Apoptosis Based on the IRE1α-XBP1 Signaling Pathway and the Protective Effect of Quercetin. Toxics. 10.3390/toxics13020129
Pei-pei Fang et al. (2020) ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes. Mediators of Inflammation. 10.1155/2020/8183713
Metin Tuba Ozcan et al. (2022) Expression of ER stress markers (GRP78 and PERK) in experimental nephrotoxicity induced by cisplatin and gentamicin: roles of inflammatory response and oxidative stress. Naunyn-Schmiedebergs Archives of Pharmacology. 10.1007/s00210-022-02358-5
Wei Wei et al. (2011) Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion. Environmental Toxicology. 10.1002/tox.20724
Lumei Chi et al. (2019) miR-9-5p attenuates ischemic stroke through targeting ERMP1-mediated endoplasmic reticulum stress. ACTA Histochemica. 10.1016/j.acthis.2019.08.005
