| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein; GRP-78; Binding-immunoglobulin protein; BiP; Heat shock protein 70 family protein 5; HSP70 family protein 5; Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; HSPA5; GRP78 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GRP78/BIP/HSPA5 recombinant protein (Position: D78-S607). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects endoplasmic reticulum chaperone BiP (gene HSPA5) in human, mouse and rat samples and is validated for Western blot and ELISA.
BiP (HSPA5, also called GRP78) is the main HSP70 chaperone of the endoplasmic reticulum lumen. It helps fold and assemble newly made secreted and membrane proteins, holds misfolded ones for degradation, and controls the unfolded protein response sensors IRE1, PERK and ATF6. It is a standard marker of ER stress.
| Target | Endoplasmic reticulum chaperone BiP (gene HSPA5; UniProt P11021, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human GRP78/BIP/HSPA5 fragment (Asp78–Ser607), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endoplasmic reticulum chaperone BiP in the endoplasmic reticulum lumen, melanosome, cytoplasm and cell surface.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuzhen Sui et al. (2024) BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress. CNS Neuroscience & Therapeutics. 10.1111/cns.14840
Guan Xiaoyu et al. (2024) CircSSR1 regulates pyroptosis of pulmonary artery smooth muscle cells through parental protein SSR1 mediating endoplasmic reticulum stress. Respiratory Research. 10.1186/s12931-024-02986-w
Liuxin Wang et al. (2025) Cadmium-Induced Kidney Apoptosis Based on the IRE1α-XBP1 Signaling Pathway and the Protective Effect of Quercetin. Toxics. 10.3390/toxics13020129
