| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Endoplasmin;94 kDa glucose-regulated protein;GRP-94;Heat shock protein 90 kDa beta member 1;Tumor rejection antigen 1;gp96 homolog;HSP90B1;GRP94, TRA1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of HSP90B1 (Endoplasmin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GRP94/HSP90B1 Antibody Picoband® catalog # PB9637. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively. (reported region: R43-H221).
- Molecular weight context: reported MW: 100 kDa; calculated MW: 92469 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Endoplasmin; Endoplasmin. Heat shock protein 90kDa beta member 1 (HSP90B1), known as endoplasmin, or GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. Functional note: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. . Reported localization: Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Expression/tissue context: Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. .
Research relevance and current trends
- Cancer: Researchers commonly examine how HSP90B1 (Endoplasmin) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how HSP90B1 (Endoplasmin) relates to this theme using model systems and orthogonal readouts.
- ER Proteins: Researchers commonly examine how HSP90B1 (Endoplasmin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative HSP90B1 (Endoplasmin) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of HSP90B1 (Endoplasmin) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Contains 1 HMG box DNA-binding domain.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
