| Field | Specification |
|---|---|
| Target | |
| Alternative names | GrpE protein homolog 1, mitochondrial; HMGE; Mt-GrpE#1; Nucleotide exchange factor GRPEL1; GRPEL1; GREPEL1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GRPEL1 recombinant protein (Position: Q90-A217). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets grpE protein homolog 1, mitochondrial (gene GRPEL1) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 24.3 kDa, and the supplier reports an observed band at about 24 kDa.
GrpE-like 1 (GRPEL1) is a mitochondrial nucleotide exchange factor for mitochondrial HSP70 (mortalin, HSPA9). By helping HSPA9 release ADP and rebind ATP, it keeps the chaperone cycle turning within the import motor that pulls precursor proteins through the TIM23 translocase into the matrix. GRPEL1 is studied in mitochondrial protein import and proteostasis.
| Target | GrpE protein homolog 1, mitochondrial (gene GRPEL1; UniProt Q9HAV7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 24.3 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, human HEK293 cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lymphadenoma tissue, human appendiceal adenocarcinoma tissue, human colonic adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human gall bladder adenosquamous carcinoma tissue, human gastric adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): PC-3 cells.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human GRPEL1 fragment (Gln90–Ala217), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places grpE protein homolog 1, mitochondrial in the mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
