| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutathione S-transferase A3; GST class-alpha member 3; Glutathione S-transferase Ya3; Glutathione S-transferase Yc; Gsta3; Gstyc |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse Gsta3, which shares 57.9% and 89.5% amino acid (aa) sequence identity with human and rat Gsta3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets glutathione S-transferase A3 (gene Gsta3) in mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 25.4 kDa, and the supplier reports an observed band at about 28 kDa.
Glutathione S-transferase A3 (Gsta3) is an alpha-class GST that conjugates glutathione to hydrophobic electrophiles. It is an efficient catalyst of the double-bond isomerization steps that produce precursors of testosterone and progesterone, and in mouse it shows high activity against the reactive epoxide of aflatoxin B1. It is studied in steroidogenesis and protection against toxins.
| Target | Glutathione S-transferase A3 (gene Gsta3; UniProt P30115, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 25.4 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
Samples with a confirmed band (WB): rat testis tissue, rat ovary tissue, rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells.
Immunogen
Synthetic peptide from the N-terminal region of mouse gsta3. Sequence identity with the human and rat orthologs: 57.9% and 89.5%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places glutathione S-transferase A3 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
