| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutathione S-transferase Mu 3; GST class-mu 3; GSTM3-3; hGSTM3-3; GSTM3; GST5; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human GSTM3 recombinant protein (Position: E93-Q206). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes glutathione S-transferase Mu 3 (gene GSTM3) in human and mouse samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 26.6 kDa, and the supplier reports an observed band at about 27 kDa.
Glutathione S-transferase Mu 3 (GSTM3) is a mu-class GST that conjugates glutathione to hydrophobic electrophiles. It is highly expressed in testis and brain, where it may help detoxify foreign and endogenous compounds at blood–tissue barriers. GSTM3 is studied in detoxification, oxidative stress and cancer susceptibility.
| Target | Glutathione S-transferase Mu 3 (gene GSTM3; UniProt P21266, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.6 kDa |
| Observed MW | 27 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human SiHa cells, human THP-1 cells, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human bladder cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human GSTM3 fragment (Glu93–Gln206), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places glutathione S-transferase Mu 3 in the cytoplasm. Tissue expression noted by UniProt (human): Testis and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Li et al. (2019) Revealing the pathogenic changes of PAH based on multiomics characteristics. Journal of Translational Medicine. 10.1186/s12967-019-1981-5
Zheng Zhe et al. (2024) 4D label-free proteome analysis of the liver damage mechanism in mice with chronic benzene exposure. Molecular & Cellular Toxicology. 10.1007/s13273-024-00482-2
