| Field | Specification |
|---|---|
| Target | |
| Alternative names | GTP-binding protein 4; Chronic renal failure gene protein; GTP-binding protein NGB; Nucleolar GTP-binding protein 1; GTPBP4; CRFG; NOG1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GTPBP4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21G64 is a rabbit monoclonal antibody that recognizes GTP-binding protein 4 (gene GTPBP4) in human samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 74.0 kDa, and the supplier reports an observed band at about 80 kDa.
GTP-binding protein 4 (GTPBP4, NOG1) is a nucleolar GTPase that takes part in building the 60S large ribosomal subunit. It has also been reported to restrain p53 stabilization, linking ribosome biogenesis to cell cycle control. GTPBP4 is studied in ribosome assembly and cancer cell growth.
| Target | GTP-binding protein 4 (gene GTPBP4; UniProt Q9BZE4, human) |
|---|---|
| Clone | 21G64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 74.0 kDa |
| Observed MW | 80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GTPBP4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places GTP-binding protein 4 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
